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Subsequently, cells were washed with PBS and pre-extracted in CSK buffer [25 mM HEPES (pH 7.7), 50 mM NaCl, 1 mM EDTA, 3 mM MgCl 2 , 300 mM sucrose, 0.5% (v/v) Triton X-100] for 10 min at RT

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Among these disorders, goiter is commonly observed in the general population and can be presented as either diffuse or nodular types based on thyroid histology