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In the first, vessel density rose in a chick chorioallantoic membrane assay and endothelial tube formation rose in vitro.[6] In rats with an ischaemic hind limb, blood flow recovered faster, measured by laser Doppler scanning.[6] Histology of that muscle showed more vessels and stronger vascular expression of VEGFR2.[6] In cultured human vascular endothelial cells, VEGFR2 mRNA and protein rose, but VEGF-A did not.[6] The authors link this to VEGFR2 internalisation and the VEGFR2-Akt-eNOS pathway, noting that both tube formation and pathway activation were suppressed by dynasore, an endocytosis inhibitor.[6] An earlier study, working with cell culture alongside crushed and transected rat muscle and tendon, reported no direct angiogenic effect on cell cultures.[7] In the treated animals, immunohistochemistry showed modulated angiogenesis with up-regulated VEGF expression.[7] So the animal results agree more closely than the cell-culture ones, and the disagreement between the two cell-culture findings is not resolved within the cited work