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We first assessed the expression of REST in different organs and found moderate expression in the heart, liver, spleen, lung, and kidney, as well as lower levels in the brain (Supplemental Figure 1, A and B

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For the negative geotaxis test, the time required for the rat to perform a 180° turn when placed head-down on a 25° inclined slope from PN 7 to PN 10 was recorded, with a 60-s time threshold