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Abbreviations Used BAL bronchoalveolar lavage CySS cystine DNTB 5,5′-dithiobis-(2-nitrobenzoic acid) ELF epithelial lining fluid FAD flavin adenine dinucleotide GPX glutathione peroxidases GR glutathione reductase GRX glutaredoxins GSH glutathione, reduced (thiol) form GSNO nitrosoglutathione GSSG glutathione disulfide GST glutathione-S-transferase H 2 O 2 hydrogen peroxide HPLC high-performance liquid chromatography IL interleukin MetO methionine sulfoxide MMA mild-to-moderate asthma NF-κB nuclear factor kappa B • NO nitric oxide radical NO 2 − nitrite NO 3 − nitrate Nrf2 nuclear factor (erythroid-derived 2)-like 2 O • 2 − superoxide anion ONOOH peroxynitrous acid OONO- peroxynitrite PRX peroxiredoxins RANTES regulated on activation, normal T-cell expressed and secreted RNS reactive nitrogen species ROS reactive oxygen species SA severe asthma -SeH selenocysteine (selenol) residue SeOH selenenic acid SeSG selenenyl sulfide adduct -SG reactive glutathione -SH sulfhydryl SOD superoxide dismutase TGFβ1 transforming growth factor beta-1 Th2 T helper type 2 TRX thioredoxin Acknowledgment This work was supported in part by RO1 NR012021

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Step 3: Limit Multi-Dose Vial Access and Maintain Sterile Withdrawal Technique Every needle puncture through the vial septum introduces three risks: microbial contamination, particulate shedding from the rubber stopper, and air exchange that accelerates oxidation