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In another study that evaluated the effect of 6 g/m 2 (i.e., dosage based on body surface area) twice daily glutamine versus placebo (three weeks followed by three weeks washout) in 49 children and adolescents receiving vincristine for various cancers, glutamine-treated patients reported significantly greater improvements in sensory function and quality of life compared with placebo-treated patients (Sands 2017)

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Cell debris was removed by centrifugation at 4400 g at 4 °C for 30 min and the clarified cell lysate was used for protein purification