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3.10 O-glycosylation at T323 and S325 of RBD (319-541)-His6 detected with ESI-MS Out of the six potential O-glycosylation sites in SARS-CoV-2 RBD, only one site showed significant occupancy in RBD (305-543) expressed in HEK-293T cells ( We confirmed the presence of O-glycosylation at the N-terminus of RBD (319-541)-His6 at T323 or S325 in all six analyzed batches, three of which were from RBD monomers and three from RBD dimers

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Peak purity test To verify the purity of the Nɛ-Hcy-Lys peak on a chromatogram of a plasma sample we have used the peak purity test and also we have analyzed samples of the standard solution of several medicinal substances containing a thiol group or a disulfide bound in their structure

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