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The experiments were performed in a buffer solution containing 20 mM HEPES (pH 7.0) and 150 mM NaCl

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While this allowed us to examine the impact of circulating factors associated with an obese intrauterine environment, it limited our ability to examine how other maternal factors, such as maternal gestational diabetes mellitus (GDM), interact with maternal obesity to influence offspring risk for MASLD and limits the generalizability of our findings to other patient groups