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The amount of formazan that is generated corresponds to the number of live cells [49] In brief, SH-SY5Y cells were seeded at a density of 5 × 10 4 in a 96-well plate (6 wells/concentration group plus 1 control group) and, following various treatments, cells were incubated with 20 μL of MTT stock solution (5 mg/mL in PBS 1X) in 180 μL of medium in the dark

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Vincent’s Hospital, Sydney and previously served, by ministerial appointment, as a Member of the Aged Care Quality and Safety Council