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This process begins with the oxidation of 5-methylcytosine to 5-hydroxymethylcytosine, followed by further oxidation to 5-formylcytosine and 5-carboxylcytosine, with the oxidized intermediates eventually removed through the base excision repair mechanism, restoring the DNA to an unmethylated state

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Copper (Cu) is an essential trace element and the third most abundant trace metal after iron and zinc in the human body (Barceloux, 1999)