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✅ Pyrogen testing is widely used across biopharma and medical devices to detect fever-causing substances before a product reaches the patient ✅ Replacing animal-based testing with MAT means broader contaminant detection, greater accuracy, and a more ethically defensible workflow as recorded under general chapter 2.6.30 ✅ MAT uses small volumes of standardized human cell cultures and cytokine release to quantify contamination, facilitating a clearer, more sensitive readout ➡️ The reliance of MAT on living human cells is both its advantage and its biggest challenge

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[DOI] [PMC free article] [PubMed] [Google Scholar] 175.Słomczyńska M, Szołtys M, Duda M, Sikora K, Tabarowski Z