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While increased permeability sounds beneficial, it can actually lead to irritation if the copper peptide concentration reaches deeper layers faster than intended
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First, construct a calibration curve using the kit’s 7 pre-calibrated rat GSH-PX standards (0.1–10 U/mL) and fit with a four-parameter logistic (4PL) regression (R² ≥ 0.995 is mandatory for academic publications)—linear regression underestimates low and high activity values
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