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6), S17–S37 (2003)
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The supernatant was passed over Ni-NTA agarose (Qiagen) and the protein was eluted in elution buffer (50 mM Tris-HCl pH 8.0, 300 mM NaCl, 10% glycerol, 250 mM imidazole and 1 mM DTT)
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