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Briefly, following acetone precipitation and resuspension in digestion buffer, protein samples corresponding to 20 µg in 20 µL were spiked with AQUA peptides into each sample at a concentration of 20 fmol per 1 µg of estimated protein content prior to digestion

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Funding This work was supported by the China Animal Husbandry Group (DR201905) and the 2115 Talent Development Program of China Agricultural University (2115)