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Contrast-to-mass calibration was performed using a native protein marker (NativeMark unstained protein standard, Thermo Scientific) with approximately 350-fold dilution in the MP buffer (50 mM HEPES-NaOH pH 8.0, 300 mM NaCl, filtered through a 0.2-µm filter)

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The reason for this finding is not clear but it is very possible that, such as the case with folate, some individuals with ASD may have a defect in the transport mechanism for B12 into the brain